What is 2-mercaptoethanol (BME)?
Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals.
How are Monothioacetals prepared from 2-mercaptoethanol?
Monothioacetals are commonly prepared from 2-mercaptoethanol using strongly acidic conditions. Various catalysts are reported that avoid these harsh conditions. These catalysts are used with 2-mercaptoethanol or propanol and are highly selective for aldehydes over ketones.
What is GIBCO 2-mercaptoethanol?
Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, so most protocols require daily supplementation.
How is protein disulfide reduced with 2-mercaptoethanol?
The reduction of protein disulfides with 2-mercaptoethanol proceeds rapidly via a two-step process involving an intermediate mixed disulfide ( Figure 2.76 ). Due to its strong reducing properties, the reagent is used most often when complete disulfide reduction is required.
How is 2-mercaptoethanol exposed to the general population?
Occupational exposure to 2-mercaptoethanol may occur through inhalation and dermal contact with this compound at workplaces where 2-mercaptoethanol is produced or used. Monitoring data indicate that the general population may be exposed to 2-mercaptoethanol via dermal contact with this compound and other products containing 2-mercaptoethanol.
Does mercaptoethanol have a negative inotropic effect?
The negative inotropic effect produced by low concentration of mercaptoethanol on isolated frog heart was blocked by pretreatment with atropine. 2-Mercaptoethanol induced arteriosclerosis and endothelial cell cytotoxicity in baboons were inhibited by sulfinpyrazone.